Afroza Parvin
University Of Manitoba, Canada
Title: Understanding the role of Antibiotic and heavy metal resistant-Enterobacteriaceae in the textile industrial effluents
Biography
Biography: Afroza Parvin
Abstract
In this present study, both lactose and non-lactose fermenting bacterial isolates were isolated from five different textile industrial canals of Bangladesh in 2016. Total bacterial colony (TBC) was counted for day 1 to day 5 for 10-6 dilution to 10-10 dilution. All the isolates were isolated and selected using 4 differential media, and tested for minimum inhibitory concentration (MIC) of heavy metals, and antibiotics susceptibility estimation. Binary exposure experiment, and plasmid profilings were also done. The colony forming units (CFU) per plate were countable mostly for 10-10 dilution. A total of 50 Shigella, 50 Salmonella, and 100 E.coli (Esherichia coli) like bacterial isolates were selected for this study where the MIC was ≤ 0.6 mM for 100% Shigella and Salmonella like isolates, however, only 3% E. coli like isolates had the same MIC for nickel (Ni). The MIC for chromium (Cr) was ≤ 2.0 mM for 16% Shigella, 20% Salmonella, and 17% E. coli like isolates. Around 60% of both Shigella and Salmonella, but only 20% E.coli like isolates had a MIC of ≤ 1.2 mM for lead (Pb). The most prevalent resistant pattern for azithromycin (AZM) for Shigella and Salmonella like isolates was found 38% and 48%, respectively, however, for E.coli like isolates, the highest pattern (36%) was found for sulfamethoxazole-trimethoprim (SXT). In the binary exposure experiment, antibiotic zone of inhibition was mostly increased in the presence of heavy metals. The highest sized plasmid was found 21 Kb and 14 Kb for lactose and non-lactose fermenting isolates, respectively.